Abstract
Parasexual hybrid callus ( Nicotiana glauca + N. langsdorffii) was separated into eight pieces by marking the relative position of each piece in an original callus, and Fraction I protein in each piece was analyzed by isoelectrofocusing. The results showed that the ratios of two kinds of large subunits, which are coded in chloroplast DNA, are completely different in different positions in the callus. This evidence demonstrates that a hybrid callus is a three-dimensional mosaic of cells containing different ratios of two kinds of chloroplasts. The Fraction I protein of five sub-cloned calli grown from single cells isolated from the hybrid callus was analyzed. Two calli had only the N. langsdorffii type of large subunits and one callus had only the N. glauca type. However, two calli contained both types of large subunits, showing that some cells in the original hybrid callus contained chloroplasts from both sources.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
