Studies on the mobilization of a maize transposable family,Ac/Ds, in pepper using In Vivo transient assay system

Abstract

A maize transposable family, Ac/Ds, has been successfully utilized as a mutagenizing agent not only in monocot but also in dicot. In order to develop insertional mutagenesis system in pepper, the mobility of Ac/Ds has been examined. In this study, the excision of the elements was monitored via transient assay system with protoplasts. Two different systems were developed and compared; one- and two-elements systems. In a one-element system, Ac alone was introduced into cells. As a two-element system, Ac and Ds were cloned into a single vector and were expressed in protoplasts. Our data showed that both Ac and Ds elements were highly mobile in pepper cells. This is the first report suggesting that Ac/Ds mediated gene tagging system could be successfully operated in pepper.