Abstract
Germfree allogeneic bone marrow chimeras (ABMC) were produced by the intravenous injection of approximately 10 7 bone marrow cells from DBA 2 mice into lethally irradiated C3H mice. In the germfree state, the ABMC showed no histological signs of graft-versus-host reactions (GVHR), and a normal lifespan was attained. Splenic lymphocytes of the germfree ABMC were shown to be unable to respond with DNA synthesis to the T-cell mitogens PHA and Con A, as measured by tritiated-thymidine ( 3H-TdR) uptake, but 7 months following bone marrow transplant, they abruptly regained the ability to respond normally to these mitogens. The plaque-forming cell response to sheep red blood cells in vivo, however, remained very low throughout the lifespan of the ABMC and actually declined at the same time as the mitogen responses returned to normal. It was inferred that helper T cells are not required for T-cell-dependent mitogen responsiveness.
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