Abstract
(1) A new bacteria was isolated from acetone-butanol fermentation broth which showed an unanticipated formation of isopropanol. It was identified that this microorganism is a variety of brevis and was named Lactobacillus brevis var. hofuensis nov. var.(2) brevis var. hofuensis produces isopropanol as the result of the reduction of acetone by its addition to medium.(3) Cultured with Clostridium acetobutylicum, brevis var. hofuensis reduces the acetone which is produced by the former organism, and furthermore, Clostridium converts the acids to solvents.(4) The oxidation-reduction between acetone and isopropanol was demonstrated by the intact cells of brevis var. hofuensis.(5) The activity of acetone reduction by brevis var hofuensis was compared with other microorganisms and the reducing potency was proved.(6) Specificities of brevis var. hofuensis for alcohols, polyols and other similar compounds were investigated.(7) Alcohols, glycols, glycerol, and mannitol, especially secondary alcohols and polyols from C2 to C6 were readily oxidized.(8) Methanol, aromatic alcohols, hydroxy acids and substitutes of alcohols did not undergo reaction.
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