Abstract
AbstractA useful system was developed for studies on the mechanism of uptake of liposomes by isolated perfused liver. The uptake of multilamellar vesicles (MLV) composed of hydrogenated egg phosphatidylcholine (HEPC), cholesterol and dicetylphosphate (ratio, 5:4:1) by perfused rat liver was enhanced by preincubation with serum. This opsonic activity decreased with increase in the liposome concentration, suggesting the depletion of opsonins. Saturation of hepatic uptake without depletion of opsonins on increasing the liposome concentration was also demonstrated. Liposomes of this composition were shown to activate the complement system in an in vitro release study using 6(5)-carboxyfluorescein (CF), so their uptake via complement receptor was examined after pretreatment of the serum with heat (56°C, 30 min) or antiC3-antiserum. The opsonic activity was completely inhibited by these treatments, suggesting complement receptor-mediated phagocytosis. K76COOH, an inhibitor of C5a formation, also inhibited the o...
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