Abstract
Yields of infectious pancreatic necrosis virus from fathead minnow cell cultures were maximal at 20 degrees C. The virus failed to replicate at 28 degrees C and neither virus-specific mRNA nor virus-specific polypeptides could be detected when infected cells were maintained at this temperature. Intrinsic thermolability of virus infectivity or inability to adsorb to cells at 28 degrees C could not account for the temperature-dependent block in virus morphogenesis. Analysis of infectious virus production and virus-specific polypeptide and RNA synthesis following shifts from the permissive (20 degrees C) to the non-permissive temperature (28 degrees C) at various times after infection indicated that multiple temperature-sensitive (ts) steps were involved in the inhibition of virus replication.
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