Abstract
Abstract The equilibrium exchange of hydrogen between the 2,3-methylene positions of succinate and water was studied under aerobic conditions with specifically tritiated and deuterated succinates and soluble succinic dehydrogenase prepared by the method of Singer from bovine heart. The exchange of tritium from racemic 2-tritiosuccinate (racemic-T1), racemic 2,3-ditritiosuccinate (racemic-T2), and meso-2,3-ditritiosuccinate (meso-T2) proceeds to completion and exhibits simple, first order kinetics. The rates of exchange of tritium from racemic-T1 and racemic-T2 are identical and 1.35 times faster than that from meso-T2. The enantiomorphs of racemic-T1 and racemic-T2 are not distinguished kinetically. Hydrogen exchange products of deuterated succinates were identified by their infrared spectra. In the exchange of deuterium from 2,2,3,3-tetradeuterosuccinate (D4) to water, meso-2,3-dideuterosuccinate (meso-D2) is the only major intermediate detected between D4 and the final product, succinate. 2-Deuterosuccinate is detected as an intermediate during the exchange of deuterium from racemic 2,3-dideuterosuccinate, but not from meso-D2. In the exchange of deuterium from D2O to succinate, meso-D2 is again the major intermediate detected between succinate and the final exchange product, D4. The exchange of deuterium from succinate to water goes to completion regardless of the extent and stereochemistry of deuteration. Hydrogen exchange proceeds mainly through the exchange of trans (RS) pairs of methylene hydrogens, and is analogous to the trans elimination of a proton pair during the oxidation of succinate to fumarate. Kinetic isotope effects appear to relate only to the nature of trans hydrogen pairs and to be unrelated to the stereochemical differences between labeled enantiomorphs. Activation of succinate seems to involve both hydrogens of a trans pair symmetrically and thus all 4 methylene hydrogen atoms are very nearly equivalent kinetically.
Highlights
Our studies indicate that the exchange of hydrogen between succinate and water, as catalyzed by the soluble succinic dehydrogenase of Singer from bovine heart, occurs mainly with truns (RS) specificity and involves 2 hydrogen atoms per event
D), and heart muscle particles was interpreted by Gawron, Glaid, and Francisco [15], as a positional nonequivalence among the methylene positions imposed by succinic dehydrogenase
In the exchange of deuterium from 2,2,3,3-tetradeuterosuccinate (D4) to water, meso-2,3-dideuterosuccinate (meso-DB) is the only major intermediate detected between Dq and the fmal product, succinate. 2-Deuterosuccinate is detected as an intermediate during the exchange of deuterium from racemic
Summary
ReagentsTritiated succinates (New England Nuclear) were prepared by the catalytic hydrogenation of suitable olefins in ethyl acetate: racemic 2-tritiosuccinate (racemic-T,)’ from maleic anhydride and TH gas (produced by the reaction of carrier-free T,O with lithium aluminum hydride) ; racemic 2,3-. Ditritiosuccinate (racemic-Tz) from carrier-free tritium gas and fumaric dimethyl ester; meso 2,3-ditritiosuccinate (meso-Ts) from carrier-free tritium gas and maleic anhydride. The cornpounds were received at specific activities of 154, 85, and 107. C. Hollocher mC per mmole, respectively, and were routinely diluted 10. QO-fold further with cold succinate for exchange esperiments Hollocher mC per mmole, respectively, and were routinely diluted 10. or QO-fold further with cold succinate for exchange esperiments
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