Abstract
The mechanism of blackspot development in Norway lobster ( Nephrops norvegicus) was studied. A linear relationship was observed between phenolase activity and rate of colour development in homogenates. It was found that tyrosine could significantly influence the rate of colour development. Production of dihydroxyphenylalanine (DOPA) during colour development was demonstrated using an HPLC method. The production of DOPA, i.e. tyrosinase activity, and the rate of colour development in homogenates of Norway lobster were found to be directly related to the phenolase activity as measured using catechol oxidase activity. It is proposed that tyrosine is the initial substrate for blackspot development in Norway lobster and that the process is an enzymatically controlled oxidation. It is suggested that phenolase from Norway lobster is tyrosinase (EC.14.18.1) which has both monophenolase and diphenolase activity.
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