Abstract
The topical carcinogenicity to mouse skin of smoke condensates obtained from a tobacco substitute (NSM), alone or in combination with tobacco, has been compared with condensate from tobacco and with acetone, the solvent used. Sixteen different types of cigarette were used to make the condensates, and the age-standardized results have been analysed according to the Weibull distribution model. The results show that NSM condensate has less than 25% of the potency of tobacco condensate (37% at 95% upper confidence limit), and that condensates from blends of NSM and tobacco are similarly reduced in activity. General pathology analysis failed to reveal abnormalities due to NSM.
Highlights
THE chemical complexity of cigarette smoke precludes the precise attribution of the known carcinogenic effects to a single component or group of components, and animal models are required to assay carcinogenic activity
A model commonly used is the topical application of smoke condensates to mouse skin, and measurement of the incidence of tumours which result
The method has been much used to monitor attempts at reduction of the carcinogenicity of tobacco smoke, and there is as yet no evidence equating mouse skin response with the human lung response, it is assumed that a direct relationship exists between the ability to induce tumours in mouse skin and in the human bronchial tract
Summary
Cigarette8.-These were 2 sizes, each 70 mm long, but either 23-0 or 25-4 mm in circumference (designated A and B respectively). The yields contained 80 cages, and a total of 16 racks was of condensate were expressed in terms of mg used for these experiments, 8 racks being of fresh anhydrous smoke (FAS) (Table I). An aliquot of the condensate from each batch each unit was varied within each room to of cigarettes was evaporated under reduced avoid environmental bias from ventilation, pressure on a water bath at 40°C until con- light and temperature. The condensates were prepared at weekly the groups They were maintained on intervals and stored at room temperatures pasteurized mouse cubes The condensate preparations were applied The start was staggered over 13 weeks with an " ARH continuous pipetting unit " The experiment was divided to allow equal
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
