Abstract
The interaction of cationic dyes, pinacyanol chloride, acridine orange and safranin O, with Klebsiella O3 lipopolysaccharide has been investigated by spectrophotometric and spectrofluorometric measurements. A strong metachromasy is induced by the acidic polysaccharide in pinacyanol chloride as revealed from the appearance of the blue-shifted absorption band at 495 nm from 600 nm. The stoichiometry of the polymer/dye in the metachromatic compound, the thermodynamic parameters of interaction and the effects of different cosolvents on the stability of complex have been studied. Quenching of fluorescence of acridine orange and safranin O by the lipopolysaccharide has also been studied. The chromatropic property of the biopolymer has been established by the spectral results.
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