Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor

Shuyan Niu,Long Wang,Shusheng Zhang,Xuemei Li,Feng Li,Shiying Wang

doi:10.3390/s6101234

Shuyan Niu, Long Wang + Show 4 more

Open Access

https://doi.org/10.3390/s6101234

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Abstract

Fluorescence spectroscopy and ultraviolet (UV) spectroscopy techniques coupled with cyclic voltammetry (CV) were used to study the interaction between salmon sperm DNA and 1,10-Phenanthroline cobalt(II) complex, [Co(phen)2(Cl)(H2O)]Cl·H2O, where phen = 1,10-phenanthroline. The interaction between [Co(phen)2(Cl)(H2O)]+ and double-strand DNA (dsDNA) was identified to be intercalative mode. An electrochemical DNA biosensor was developed by covalent immobilization of probe single-strand DNA (ssDNA) related to human immunodeficiency virus (HIV) on the activated glassy carbon electrode (GCE). With [Co(phen)2(Cl)(H2O)]+ being the novel electrochemical hybridization indicator, the selectivity of ssDNA-modified electrode was investigated and selective detection of complementary ssDNA was achieved using differential pulse voltammetry (DPV).

Highlights

Nowadays, considerable attention has been paid on electrochemical DNA biosensor in the diagnosis of genetic diseases and the detection of pathogenic biological species due to the highly sensitive, rapid yet accurate, simple and inexpensive detection technique it offers [1,2,3,4,5]
Interaction between cobalt(III) complexes and DNA have been reported in many researches [15,16,17], interaction between 1,10-Phenanthroline cobalt(II) complex and DNA is seldom studied
The developed electrochemical DNA biosensor was based on the covalent immobilization of human immunodeficiency virus (HIV) probe single-strand DNA (S1) on the activated electrode

Summary

Introduction

Considerable attention has been paid on electrochemical DNA biosensor in the diagnosis of genetic diseases and the detection of pathogenic biological species due to the highly sensitive, rapid yet accurate, simple and inexpensive detection technique it offers [1,2,3,4,5]. Researches conducted showed that transition metal complexes have important biological activities. In order to obtain more insight into the design of highly sensitive reactive probes, diagnostic reagents and new drugs, detailed understanding of the interaction between such complexes and DNA is potentially useful. After probe single-strand DNA (ssDNA) related to human immunodeficiency virus (HIV) was covalently immobilized on the activated glassy carbon electrode (GCD), an electrochemical DNA biosensor using [Co(phen)2(Cl)(H2O)]+ as the electrochemical hybridization indicator was developed and it demonstrated potential in selective detection of the complementary ssDNA. The work might bring further insight on the interaction mechanism between transition metal complexes and DNA and be helpful for further research for designing novel anti-tumor drugs and diagnosis disease

Instrumentation

Reagents

Preparation of the electrochemical DNA sensor

Results and Discussion

The electrochemical characterization of modified GCE

The selectivity of the DNA electrochemical biosensor