Abstract
Fatty acid synthetase enzymes were purified from the liver, mammary gland, and adipose tissue of rats and the liver and mammary gland of mice. The enzymes from the liver and mammary gland of the same species have similar molecular weights and and dissociate into subunits at comparable rates. Rabbit antisera were prepared against the fatty acid synthetase from the lactating rat mammary gland. Cross-reactivity between different fatty acid synthetases was determined by immunodiffusion and immunoprecipitin tests. No differences in immunological cross-reactivity could be detected in liver, mammary gland, and adipose enzymes from the same species; fatty acid synthetases from the rat and mouse gave reactions of incomplete identity. Partially purified fatty acid synthetases from pigeon liver and rabbit mammary gland did not react with the antiserum. It is concluded that the immunochemical approach is useful in determining the degree of resemblance between fatty acid synthetases from different species. Within a given species, the liver and mammary gland fatty acid synthetases seem to be very similar, if not identical, proteins.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
