Abstract
The objective of this paper is to study the chemical constituents of Radix Glycyrrhizae and to apply the resulting natural products in the study of drug susceptibility of hepatoma cells so as to provide a scientific basis for quality standards and clinical application of medicinal Radix Glycyrrhizae. Chromatographic materials were used for isolation and purification; structural identification was performed based on physicochemical properties and spectral data. MTT colorimetry was used to detect the proliferation inhibition rate against primary hepatoma cells by natural products, and flow cytometry was used to detect the changes in cell cycle progression. Five compounds were isolated and identified, namely, liquiritigenin (1), liquiritin (2), isoliquiritigenin (3), betulinic acid (4), and oleanolic acid (5). In the study, 5-FU (5-fluorouracil) is used as a positive control to the hepatoma cells. Primary hepatoma cells were highly susceptible to 5-FU and liquiritigenin, both of which markedly inhibited the proliferation of hepatoma cells; flow cytometry results showed an increase in G0/G1 phase cells, a decrease in S phase cells, and a relative increase in G2/M phase cells. Primary hepatoma cells are highly susceptible to liquiritigenin, a natural product; the testing of tumor cell susceptibility is of important significance to the improvement of therapeutic effect of cancer.
Highlights
Radix Glycyrrhizae is a legume, which is recorded in the Chinese Pharmacopoeia 2010 Edition Vol I as the root and rhizome of Glycyrrhiza uralensis Fisch., G. inflata Bat., or G. glabra L. [1]
Hepatoma tissues obtained from surgical resection were used to prepare and culture primary hepatoma Journal of Chemistry cells, which were used to perform anticancer drug sensitivity test, with the aim of improving drug efficacy and reducing side effects
Comparing with 5-FU, liquiritigenin had apparent proliferation inhibitory effects on primary hepatoma cells, while liquiritin and isoliquiritigenin had relatively low sensitivities to hepatoma cells, and betulinic acid and oleanolic acid had no clear effect on these cells. 5-FU and liquiritigenin could significantly inhibit hepatoma cell progression from G1 phase to S phase, where G0/G1 phase cells increased, S phase cells decreased, and G2/M phase cells relatively increased
Summary
Radix Glycyrrhizae is a legume, which is recorded in the Chinese Pharmacopoeia 2010 Edition Vol I as the root and rhizome of Glycyrrhiza uralensis Fisch., G. inflata Bat., or G. glabra L. [1]. Despite extensive studies on chemical constituents of Radix Glycyrrhizae, pharmacological studies on monomeric compounds, especially the studies on anticancer activities and drug susceptibility, are still few [5]. To further improve the quality evaluation of Radix Glycyrrhizae and to better guide clinical medication, a systematic study of chemical constituents in Radix Glycyrrhizae is necessary, in order to obtain monomeric compounds and to study their antihepatoma activities. Domestic scholars have used this model to screen out baicalin, cordycepin, melittin, ampelopsin, ligustrazine, and other natural active constituents with relatively high inhibitory activities on hepatoma Bel-7402 cells from natural medicines and explored their application values in adjuvant therapy, susceptibility, and other aspects of liver cancer [6, 7]. Hepatoma tissues obtained from surgical resection were used to prepare and culture primary hepatoma
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