Abstract
Prymnesin, the purified toxin of Prymnesium parvum Carter, was subjected to a variety of inactivation procedures: Irradiation by visible or ultraviolet light, incubation at 37° and exposure to alkaline media. Inactivation was measured by three assay methods: Lethality for fish, hemolysis of rabbit erythrocytes, and inhibition of the acetylcholine-induced contractions of the small intestine of the guinea pig. It was observed that the rate of destruction of the antispasmodic activity exceeds by far the rate of decline of the ichthyotoxic or hemolytic potency. The heterogeneity of prymnesin is thus established.
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