Studies on the heterogeneity of fibrinolytic activity in artificially induced intravascular clots.

Abstract

SUMMARY This study was designed to investigate the effectiveness of enhancing local fibrinolytic actvity in intravascular clots preventing side reactions resulting from fibrinolytic activation in the circulating blood. For this purpose, the following procedures were performed. Artificial intravenous clots were induced in femoral and external jugular veins of dogs by three different methods. To dogs at various time intervals after clot formation, EACA or trans·AMCHA was intr a venously injected following with simultaneous human serum, and then various units of streptokinase or urokinase were intravenously administrated. Fibrinolytic activity of these clots after treatment was assayed according to the fibr in plate method, at the same time fibrinolytic activity of a thrombosed vein was assayed. The results of these experiments were as follows: 1) Artificially induced intravenous clots showed no fibrinolytic activity except thrombin-induced clots. 2) Activa tor activity of one hour to six day old clots treated with EACA or trans-AM CHA, was significantly enhanced by the simultaneous injection of strep­ tokinase or urokinase. 3) Activator activity of intima of a thrombosed vein was also enhanced with streptokinase or urokinase. 4) The administration of EACA or trans-AMCHA did not sacrifice the fibr­ inolytic activation in an intravenous clot with streptokinase or urokinase. 5) A fibrinolytic mechanism in an intravascular clot showed different charac­ terist ics from that of the circulation.