Abstract
A preliminary study of the growth of root hairs of collards in calcium nitrate, reported in No. II of this series,' represented an attempt to analyze the effect produced by increasing the concentration of the salt in the culture solution. The two separable factors in evidence were the chemical effect of the salt and the osmotic effect of the solution. In a solution containing 0.003 M sucrose, in addition to the optimum concentration of calcium nitrate, there was the same amount of retardation in growth that was obtained through the addition of an equimolar concentration of calcium nitrate. If, however, these additions were made to suboptimal concentrations of calcium nitrate, there was a decrease in the percentage of retardation due to sucrose and a positive acceleration upon the addition of equimolar amounts of nitrate. It seemed probable therefore that the effect of osmotic pressure alone was retardation, but that in this latter instance it had been overcome by the stimulative property of the calcium nitrate up to a certain concentration.
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