Abstract

SUMMARY: Membrane glycerol teichoic acid has been extracted from Lactobacillus fermenti NCTC 6991 by two different procedures. Phenol extraction gave a complex of teichoic acid with glycolipid and phospholipid (lipoteichoic acid). Trichloracetic acid extraction resulted in degraded lipoteichoic acid and free teichoic acid chains. The teichoic acid is a 1:3 phosphodiester linked glycerophosphate polymer substituted with D-alanine, D-galactose and a disaccharide of D-galactose and D-glucose. The significance of lipoteichoic acid as a membrane constituent is discussed.

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