Abstract
The free sugars of ten samples of ungerminated barley were separated by partition chromatography and estimated by the Somogyi micro-copper method. The absolute quantities of the different sugar fractions varied widely from sample to sample, but sucrose was always the most plentiful (mean values, 0.92% of grain, 49% of total free sugars) followed, in order of abundance, by raffinose and glucodifructose. Hexoses amounted to less than 10% of the total sugars. In a sample of Spratt-Archer barley, approximately 75% of the sucrose and 80% of the raffinose was located in the germ and aleurone, whereas 65% of the hexose sugar was in the endosperm.
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