Abstract
The effect of different starch and nitrogen sources on the production of amylase by the highly amylolytic isolates Bacillus subtilis BS5 from the gut of Amitermes evuncifer Silvestri was studied. Cocoyam starch proved to be the best carbon source for the enzyme production. Highest yield of amylase was achieved at pH 6.0. Enzyme synthesis occurred at 20 to 55 o C with an optimum at 50 o C. Amylase production was stimulated by Ca 2+ , Na + and Mg 2+ but inhibited by EDTA and HgCl 2 . // //
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