Studies on the effect of phenylbutazone and salicylate on the rates of synthesis of albumin and acute-phase α1-acid glycoprotein by rat liver slices

Abstract

Rates of synthesis of albumin and α 1-acid glycoprotein were determined in presence of phenylbutazone and salicylate using liver slices prepared from control and experimentally inflamed rats. The rates of synthesis in control and experimental liver slices were 50 and 35 μg/g wet wt slices per hour, respectively, for albumin and 9.5 and 24 μg/g wet wt slices per hour, respectively, for α 1-acid glycoprotein. There were significant reductions in synthesis rates of both proteins in presence of concentrations of 0.5 m m or greater of phenylbutazone or concentrations of 2 m m or greater of salicylate. Incorporation studies showed that decreased synthesis rates were accompanied by inhibition of incorporation of radioactive precursors into albumin and α 1-acid glycoprotein and into total protein isolated from liver and incubation medium.