Abstract
Ingestion of foods that contain high levels of histamine causes clinical symptoms, namely histamine poisoning. During the course of this investigation eight biogenic amines in fish samples, including histamine, putrescine, cadaverine, tyramine, tryptamine, spermine, spermidine, and beta-phenylethylamine, were separated and quantitated by TLC technique. Many solvent systems were evaluated, with the best separation in one dimension when benzene/triethylamine (5:1) was used as a developing system, which enabled separation of histamine in addition to spermine, tyramine, and beta-phenylethylamine. However, the other four biogenic amines were not separated using this developing system. The only way to separate the eight tested biogenic amines was by using a two-dimensional TLC procedure. The running system in the first direction was benzene/triethylamine/acetone (10:2:1), and the solvent system in the second direction was benzene/triethylamine (5:1). This method provides a simple and inexpensive means to separate and detect biogenic amines in fish samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
