Studies on the defectiveness of adeno-associated virus (AAV) II. Effect of growth in CV-1 cells on the replication of adeno-associated virus

Abstract

Abstract The mechanisms by which helper viruses can promote AAV macromolecular synthesis have been investigated. The third system used was a continuous line of African green monkey kidney cells (CV-1) which is permissive for AAV when a simian adenovirus helper is used. When HSV was used as a helper, no AAV DNA, structural proteins, or IF antigens were detected, indicating the absence of HSV helper activity. HSV production and plaqueing in CV-1 cells were found to be comparable to those seen in Hep-2 cells (an epidermoid cancer line) and Vero cells (another line of African green monkey kidney cells). In both of these cell lines, HSV expresses its AAV helper functions. It was also observed that AAV had no effect on HSV replication in either Vero or CV-1 cells. Comparison of HSV protein production in CV-1 and Hep-2 cells demonstrated the absence of HSV proteins ICPO and ICP46 in the CV-1 line following the release of a cycloheximide block.