Abstract
Cellular production of ribosomes involves the formation of highly defined interactions between ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs). Moreover in eukaryotic cells, efficient ribosome maturation requires the transient association of a large number of ribosome biogenesis factors (RBFs) with newly forming ribosomal subunits. Here, we investigated how r-protein assembly events in the large ribosomal subunit (LSU) rRNA domain II are coordinated with each other and with the association of RBFs in early LSU precursors of the yeast Saccharomyces cerevisiae. Specific effects on the pre-ribosomal association of RBFs could be observed in yeast mutants blocked in LSU rRNA domain II assembly. Moreover, formation of a cluster of r-proteins was identified as a downstream event in LSU rRNA domain II assembly. We analyzed in more detail the functional relevance of eukaryote specific bridges established by this r-protein cluster between LSU rRNA domain II and VI and discuss how they can support the stabilization and efficient processing of yeast early LSU precursor RNAs.
Highlights
Ribosomes play a key role in cellular gene expression by catalyzing the translation of mRNA into proteins
We reasoned that focusing on the characterization of early to intermediate nuclear large ribosomal subunit (LSU) precursor populations in our analyses should help to reveal specific compositional changes directly related to early pre-ribosomal RNAs (rRNAs) maturation defects observed in the selected r-protein expression mutants
In the present work we identified formation of an r-protein cluster at the interface of LSU rRNA domain II, ES7 and LSU rRNA domain VI (“dII/dVI cluster”) as a downstream event in the yeast LSU rRNA domain II assembly pathway important for early steps of LSU rRNA processing
Summary
Ribosomes play a key role in cellular gene expression by catalyzing the translation of mRNA into proteins. In the unicellular eukaryote Saccharomyces cerevisiae (hereafter called yeast), cytoplasmic ribosomes consist of four ribosomal RNAs (rRNA) and 79 ribosomal proteins (r-proteins). These interact with each other in highly defined ways to build the large and the small ribosomal subunit (LSU and SSU, respectively) [1,2,3,4]. Yeast LSU rRNA Domain II Assembly and Competitiveness (MINECO). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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