Studies on the constituents of higher fungi of Korea (XX)

Abstract

To investigate constituents ofStrobilomyces floccopus (Fr.) Karst. andCoprinus comatus (Fr.) S.F. Gray, free and total amino acids of the two mushrooms were quantitatively analyzed by G.L.C. and an amino acid analyzer. Free amino acids were extracted from both mushrooms with ethanol. Fourteen free amino acids were detected from the ethanol extract ofS. floccopus and fifteen free amino acids fromC. comatus by G.L.C. And the dry carphopores of both mushrooms were hydrolyzed with hydrochloric acid and then the total protein amino acids were analyzed by A.A.A. Seventeen total amino acids were detected from each acid-hydrolysate ofS. floccopus andC. comatus. Lipids were extracted from the carpophores ofS. floccopus and saponified with alcoholic potassium hydroxide. The isolated sterols were subjected to G.L.C. and two sterols were detected. The isolated free fatty acids were methylated with diazomethane and subjected to column chromatography and G.L.C. Eleven saturated and nine unsaturated free fatty acids were detected from the carpophores ofS. floccopus. The presence of these nutrient components shows that the two mushrooms can be utilized as edible ones.