Studies on the Chromosomes of Murine Dalton's Lymphoma Cells and the Effect of Cisplatin on Chromosomes of These Cells and Bone Marrow Cells in vivo.

Abstract

Chromosome studies of Dalton's lymphoma (DL) revealed only one rb-marker in the cells and the chromosome numbers varied between 59 and 68 with a peak at 66 chromosomes. Thus, the modal number of chromosomes of this DL is suggested to be 66. Cisplatin treatment of tumor bearing hosts resulted the regression of the tumor and induced the formation of chromosomal aberrations/aberrant metaphases (80-90%) in the DL cells which may be responsible for the anticancer activity of cisplatin. Cisplatin treatment of the hosts caused the significant decrease in GSH levels in DL as well as bone marrow cells. Cisplatin's mutagenic potentials in the host is also supported from the observation of the induction of chromosomal aberrations in the bone marrow cells of the same host. Differential effect of cisplatin on the chromosomes of bone marrow and Dalton's lymphoma cells is noted. Bone marrow cells developed significantly less aberrant metaphases as compared to that of DL cells after cisplatin treatment in vivo. Interestingly, aberrant metaphases in bone marrow cells decrease appreciably with time of treatment (48-96 hr) which may suggest the possibility of an efficient clearance/repair of Pt-DNA adducts in bone marrow cells but in DL cells it remained more or less constant.