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Abstract
The chemical form of Zn in the low molecular weight fraction of oyster meat was examined. The acetone-insoluble fraction (fraction C) was extracted with 50% methanol and the extract was separated into high and low molecular weight fractions by Sephadex G-50 chromatography. Starting from 40 g of fraction C, 4 g of low molecular weight fraction containing 3.6 mg of Zn was obtained. Zn in this fraction was migrated to lesser extent to cathod than Zn ion in paper electrophoresis. This Zn was eluted at the similar position to that of EDTA-Zn and 1, 4, 7, 10-tetraazacyclotridecane-Zn complex and differed also from Zn ion by siliconized pore glass column chromatography. When the low molecular weight fraction was treated with diluted acid (1 N-AcOH), liberation of Zn ion was observed both in paper electrophoresis and siliconized pore glass column chromatography. These results suggest that Zn in the low molecular weight fraction of the oyster meat occurs as a chemical form of some complexes.
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