Abstract
Pisum sativum L. (Fabaceae) is important in diet due to its fiber content, protein, starch, trace elements and phytochemical substances. Dichlorvos (2, 3-dichlorovinyl dimethyl phosphate) is commonly known as organophosphates belong to the classes of insecticides that are used to control households and stored products insects. The aim of this study was to analyze the kinetic characteristics of esterase enzyme from four different varieties of Pisum sativum L. using qualitative and quantitative method and also the docking position of esterase with Dichlorvos by insilico method. According to our study the total activity was highest for Arka Nirmala and Arka Priya varieties. The specific activity was highest for Arka Mayur variety. Km and Vmax, were found to be higher for Arka Uttam. The optimum temperature and pH for all the varieties were found to be same, 40�C and 7.5 respectively. The inhibitor studies showed Arka Nirmala was more sensitive to inhibitor. From the fitness and interaction profile it was found that the inhibitor dichlorovos is an effective pesticide which can be used to inhibit esterase activity. It has one hydrogen bond of H-S and had an amino acid residue at ARG57.
Highlights
Medicinal plants are widely used to treat some disorders and to maintain a healthy state in both modern and traditional medicine [1]
The concentration of the protein determines the quality of the Pisum sativum L. and it is strongly influenced by plant genetics and growing conditions [17]
Arka Mayur showed a slight highest specific activity of 0.008 IU/mg, which was followed by Arka Nirmala (0.006 IU/mg), Arka Uttam (0.005 IU/mg) and Arka Priya (0.003 IU/mg)
Summary
Medicinal plants are widely used to treat some disorders and to maintain a healthy state in both modern and traditional medicine [1]. It provides three main benefits: health benefits, financial benefits and society wide benefits. Many studies proved that it has antibacterial, antidiabetic, antifungal, anti-inflammatory, anti-hyper cholesterolemia, antioxidant and anticancer properties [2,3]. Plant esterases are involved in food spoilage. The significant functions of these esterases include in metabolism and subsequent detoxification of many agrochemicals and pharmaceuticals [4,5]. The esterases catalyze the hydrolysis of aliphatic and aromatic esters and have been widely studied because of their metabolic functions, in flavor development and role in the breakdown of the insecticides [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
