Studies on the cell wall of Neurospora crassa. Part V Purification and some properties of two wall-associated (.BETA.1,3)glucanases from Neurospora crassa cells.

Abstract

Two cell wall associated (β1,3)glucanases in Neurospora crassa, 1,3-glucanase-I and II, were solubilized by autolysis of the cell walls with 2 m sodium chloride, and purified by laminarioligosaccharide AH-sepharose affinity chromatography followed by preparative disc gel electrophoresis. The 1,3-glucanase-I has a strict specificity for (β1,3)glucan and produces D-glucose by an exo-type mode of action. The 1,3-glucanase-II has specificities for β-glucoside in addition to (β1,3)glucan. The 1,3-glucanase-I is more resistant to pH change and metal ions than the 1,3-glucanase-II.