Abstract
Abstract Bovine parathyroid gland slices incubated in vitro have been shown to synthesize parathyroid hormone. The incorporation of several radioactive amino acids into the hormone isolated from the tissue was found to vary inversely with the concentration of calcium present in the incubation medium. The identity of the newly synthesized hormone with parathyroid hormone was established by several lines of evidence, including a demonstration of calcium-mobilizing activity in the parathyroidectomized rat. The stimulatory effect of lowered calcium concentration was directed more to incorporation of amino acid into the hormone than into the general protein of the tissue. Examination of the incubation medium indicated significant incorporation of amino acid into soluble protein, but this was unaffected by calcium concentration. The presence of hormone in the incubation medium could not be demonstrated either by attempted isolation or by assays for biological activity.
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