Abstract
The enzyme system (culture filtrate) from Streptomyces sp. W19-1 formed gentiobiose from curdlan (β-1, 3-glucan). The mechanism of the formation of gentiobiose was investigated in this study. Two kinds of enzymes, β-1, 3-glucanase and β-glucosidase (transglucosidase), were isolated from the culture filtrate of the strain by hydroxylapatite column chromatography. The β-1, 3-glucanase hydrolyzed curdlan to glucose and laminari-oligosaccharides, and the β-glucosidase formed gentiobiose by transglucosylation from the resultant laminari-oligosaccharides, especially laminaribiose. The two enzymes took part in the formation of gentiobiose from curdlan.
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