Abstract
An intracellular nuclease, which is known to be activated about three times in the course of autolysis of Asp. oryzae, 1, 2) was partially purified in the present work. A portion of mycelia (20.6kg) obtained by submerged culture was autolyzed in 35 liters of water with 500ml of toluene at 30°C for 40hr. The nuclease was 18, 300 times purified from the filtrate of the autolyzate by chromatographies using CM-cellulose and Sephadex G-100. The DNase activity and the RNase activity were eluted at the same position in each chromatography, and the ratio of two activities was constant throughout the last steps of purification.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
