Abstract
The immunogenicities of 15 murine osteosarcomata induced with a bone seeking radioisotope ((90)Sr) in normal and chimaeric CBA mice were studied. Attempts were made to induce tumour-specific immunity in syngeneic mice by treatment with x-irradiated (15,000 rad) tumour or surgical excision of developing subcutaneous tumour grafts. Resistance was evoked against 6 tumours and this was relatively weak. With the remaining tumours, no resistance against the immunizing tumour could be demonstrated, even though the transplantation tests were made highly sensitive by the use of inocula of as few as 2 × 10(3) cells in pre-irradiated (400 rad) hosts. Sera from mice immunized against each of the tumours were tested against viable cells of the immunizing tumour by indirect immunofluorescence. In no instance did tumour antisera give a convincing reaction with tumour cells although alloantisera raised by hyperimmunization of H-2 identical and H-2 different donors with osteosarcomata consistently gave strongly positive reactions. The results are interpreted as illustrating the weak tumour specific antigenicity of radiation-induced murine osteosarcomata. The possibility that antigenic deficiency is a consequence of immunosurveillance in this tumour system is discussed.
Highlights
Summary.-The immunogenicities of 15 murine osteosarcomata induced with a bone seeking radioisotope (90Sr) in normal and chimaeric CBA mice were studied
In sarcomata induced by implanted plastic viral oncogenesis there exists, in general, films (Klein, Sjogren and Klein, 1963) a striking correspondence between malig- and pulmonary neoplasms induced by nant transformation and the appearance chemical carcinogens (Prehn, 1962, 1963; of new histocompatibility antigens, specific Pasternak, Hoffman and Graffi, 1966) for the aetiologic virus
In this paper we report on the antigenicity of osteosarcomata in mice arising as a consequence of internal irradiation with bone-seeking isotopes, by the attempted induction of resistance to their own transplantation in recipient mice, syngeneic with the strain of origin
Summary
Tumours.-The induction of osteosarcomata by injection of 90Sr (20 uCi) in normal and chimaeric CBA mice has been described (Barnes et al, 1970). With one exception (S27) primary tumours were classified histologically as osteosarcomata. One tumour in the series (Si 15) was induced by a single intraperitoneal injection of 226Ra (50 nCi). All osteosarcomata arising in syngeneic (CBA/CBAT6T6) and allogeneic (CBAT6T6/ A) chimaeras were confirmed to be of host (CBA) origin by cytological and, where appropriate, genetic analysis. Tumours were serially passaged by subcutaneous implantation in young adult male syngeneic CBA or CBAT6T6 mice, the genetic uniformity of which was routinely checked by skin grafting
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
