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Abstract
Growth-inhibitory concentrations of racemic sn-1(3)-dodecylglycerol inhibit the incorporation of [14C] glycerol into lipids and lipoteichoic acid of Streptococcus mutans BHT and alter the per cent composition of the glycerolipids. Increases in phosphatidic acid and diphosphatidylglycerol (at the expense of phosphatidylglycerol) contribute the most to the change in lipid composition. No cellular lysis occurs under these conditions. Radioactive racemic sn-1(3)-dodecylglycerol is readily taken up by the cell and is metabolized primarily to lysophosphatidic acid and phosphatidic acid with smaller amounts converted to phosphatidylglycerol and diacylglycerol. The accumulation of phosphatidic acid and the loss of viability respond in parallel to different concentrations of dodecylglycerol. An increase in CTP is also observed which together with the increase in phosphatidic acid suggests a possible impairment in the synthesis of CDP-diacylglycerol.
Highlights
Growth-inhibitoryconcentrationsofracemic sn- observed to inhibit peptidoglycan synthesis in S. fuecium l(3)-dodecylglycerolinhibit the incorporation of [’“C] ATCC 9790 and Streptococcus mutuns BHT ( 7 ),a bacterium glycerol into lipids and lipoteichoic aofciSdtreptococ- which possesses little or no autolysin activity (8, 9), but is cus mutans BHT and alter the per cent composition of readily killed by DDG
Thisbacterium is tolerant to penicillin and several other cell wall inhibiting antibiotics
There appears to be a correlation between the blockage of lipid metabolism at the level of phosphatidic acid with loss of viability, which occur at thesame concentration of dodecylglycerol
Summary
ITS LIMITED METABOLISM AND INHIBITION OF GLYCEROLIPID AND LIPOTEICHOIC ACID BIOSYNTHESISIN STREPTOCOCCUS MUTANSBHT*. Growth-inhibitoryconcentrationsofracemic sn- observed to inhibit peptidoglycan synthesis in S. fuecium l(3)-dodecylglycerolinhibit the incorporation of [’“C] ATCC 9790 and Streptococcus mutuns BHT ( 7 ) ,a bacterium glycerol into lipids and lipoteichoic aofciSdtreptococ- which possesses little or no autolysin activity (8, 9), but is cus mutans BHT and alter the per cent composition of readily killed by DDG. In contrast to penicillin treatment, preliminary experiments (13) show that exposure of exponentially growing cultures of S. mutans BHT togrowth inhibitory concentrations of DDG effectively inhibits lipid and lipoteichoic acid biosynthesis. Because of these opposing effects of DDG and penicillin on lipid metabolism and because S. mutans BHT is killedby DDG but notby penicillin, we thought itparticularly relevant to undertake this study to determine the metabolic fate of dodecylglycerol. Known for sometime, relatively little is known about how a lipid, especially at low concentrations, exerts its antibacterial
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