Abstract
1. 1. Removal of albumin from plasma using Blue Sepharose as an affinity agent was studied using blood from rats, guinea pigs, baboons and humans with the following results. 2. 2. Relative affinities of albumin obtained from these species for the affinity matrix were human = baboon > guinea pig = rat . 3. 3. Human albumin was removed with the greatest percentage recovery. SDS-polyacrylamide gel electrophoretic analysis of the several plasma fractions obtained under different conditions indicated that albumin was, in all sera, the predominant component of the bound material. 4. 4. Recovery of albumin in the unbound fraction may result from incomplete binding to the matrix due to variations in sample preparation.
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