Studies on the Actions of Calcium Channel Blockers, Anticholinesterases Agents on Experimentally Induced Spastic Paralysis in Normotensive Rats

Abstract

Experimental paralysis was induced intramuscularly (i.m) in rats , using Suxa(1.08mg/kg ) , Deca (1.08mg/kg ) in normotensive rats weighing150-180g±2.5g (n=8).Various concentrations of these drugs ranging from (0.36—1.08mg/kg) were used , in order to assess at what concentrations will induced spastic paralysis sustained without death. Similarly, dose response concentrations effect was examined and the onset of action( ie time taken to observe paralysis and manifestation of pain and death was recorded. In some experiments, equimolar concentrations of Suxa(1.08mg/kg) and Deca(1.08mg/kg) was used to assess reaction time(RT) and potency of the agents. Thereafter the actions of Calcium Channel blockers (CCB), Diltiazem ( DTZ) and verapamil(VE) were studied in separate experiments. Anticholinesterase agent(AchE) in combination with Atropine a known antimuscarinic agent(AchE ) and investigated their actions on induced paralysis. The results obtained in these studies showed that CCB produced a significant potentiation of spastic paralysis on Suxa induced paralysis but non reversal effect with Deca , while AchE in combination ATR showed a reversal effect on paralysis induced by these agents in the concentrations used in his study. The aim of the study was to see interaction of these agents on sustained experimental paralysis in rodent.