Studies on the ability of rat lung and liver microsomes to facilitate transfer and metabolism of benzo[ a]pyrene from diesel particles

Abstract

Little is known about the bioavailability of inhaled organic compounds that are associated with particles. It is known that certain particle-associated organic compounds, such as polycyclic aromatic hydrocarbons (PAH) adsorbed on diesel soot particles, are retained in the lung longer than PAH inhaled in pure form. If such particle-associated compounds are available for tissue interaction, their prolonged retention may result in an increased potential for a toxic effect. To determine the factors affecting the bioavailability of particle-associated PAH, we have studied the ability of microsomes to facilitate transfer of benzo[ a]pyrene (B[ a]P) adsorbed on the surface of diesel exhaust soot particles to the microsomes and the ability of the microsomes to metabolize the transferred B[ a]P. Our results indicate that rat lung and liver microsomes were able to facilitate the transfer of small amounts of B[ a]P from diesel particles (<3%), but only a fraction of the amount transferred (1–2%) was metabolized. Under the same incubation conditions without soot, free B[ a]P was extensively metabolized by microsomes, principally to B[ a]P-9,10-diol. Lung microsomes were about twice as effective as liver microsomes for the transfer of the B[ a]P. The ability to transfer B[ a]P to the microsomes was independent of metabolism or the presence of protein, but was related to the lipid content of the microsomal fraction. There was no metabolism of the B[ a]P coated on diesel particles as analyzed by high-performance liquid chromatography. These findings suggest that microsomes are able to enhance the slow transfer of only a small amount of B[ a]P from diesel particles in a form that can be metabolized. However, over a long period of time, this slow release might be significant.