Abstract
1. 1. The total lipid was extracted from the spermatozoa of fresh-water mussel ( Hyriopsis schlegelii) with chloroform-methanol and the extract was treated with mild alkali. The alkali-stable lipid fraction,which mainly included the sphingolipids, was fractionated on a silicic acid column. 2. 2. Five classes of sphingolipid were separated and characterized as an unidentified sphingolipid, ceramide monohexoside, ceramide 2-aminoethylphosphonate and two kinds of mucolipids. The ceramide monohexoside was composed of ceramide galactoside and a trace amount of ceramide glucoside. No sphingomyelin was found in the sphingolipids. 3. 3. The sugar moieties of the mucolipids were composed of fucose, xylose, mannose and glucose in a ratio of 1:1:2:3 and the amino sugar of the mucolipids was identified as glucosamine, while no sialic acid was detected in them. 4. 4. The fatty acid compositions of the sphingolipids were mainly composed of saturated acids, in which palmitic and stearic acids were predominant.
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