Studies on Silicified Tissues of Leaf in Rice Plant by Spodogram-Hydrogen Chloride Treatment Method.

Abstract

When studied on silicified tissue of plant by histochemical method, Grob's phenol method was used as the most popular technique. But this method did not caused by chemical reaction of silicate, silicified tissues of leaf in rice plant was not cleared enough. In this studies we separated pure silicified tissues from leaf spodogramm by adding dilute hydrogen chloride. Results are summarized as follow: 1. Figure 1 to figure 6 shows the silicate accumulating process as the growth of leaf blade. In these figures we found the dumbbell form cell was most rapidly silicified and other epidermis cells were also beginning to silicified gradually. But when the leaf blade emerges from the leaf sheath of under leaf, these cells have been silicified almost completely. 2. Silicified motor cells of matured leaf are shown in figure 7, 8, 13 and 14. Situation of motor cells in leaf blade tissue are shown in figure 9, 10 and 11. According to these figures, motor cell's square shape in figure 7 is the same as bottom line of motor cell in figure 12 (C). 3. Leaf sheath also was silicified completely as figure 15 to figure 17. Leaf sheath node was partially silicified as form as net showing in figure 18.