Abstract
A method for the preparation of a new packing material for high performance liquid chromatography by bonding an anti-interferon monoclonal antibody to the surface of silica gel is described. The high coupling efficiency and activity of the interferon-alpha A-monoclonal antibody (IFN-alpha A-McAb) column were obtained by activated diol-silica gel with an activating agent. After purification with this packing material, the specific activity of recombinant human interferon-alpha A rose by up to 1.03 x 10(7)IU/mg protein and the purification efficiency was increased by approximately 100 times.
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