Abstract
Reticulocyte ribosomes were dissociated into metabolically active subunits by suspending the crude ribosomal preparation in 0.5 m KCl at a very high ribosome concentration (above 6 mg/ml). These subunits, which were harvested by sedimentation and stored in 0.25 m sucrose, are called shocked ribosomes (s-ribosomes). Structural transformations were exhibited when the ionic environment of the s-ribosomes was altered. In 0.1 m Tris-Cl, pH 7.5 (at 23 °) a suspension of s-ribosomes at a concentration of 4 mg/ml yields two major sedimenting species with sedimentation coefficients of 30S and 47S at 20 °. The relative concentration (by weight) of these subunits. was 1–2. The appearance of these two distinct subunits species depends on the relative concentration of Tris-Cl and ribosomes, and on the time of incubation. The degree of reconstitution into particles of 72S or larger parallels that of recovery of activity for amino acid incorporation.
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