Abstract

The reactivity of XNAs in the serum samples from 18 insulin-dependent diabetes mellitus(IDDM)patients and 20 healthy persons to the pancreatic tissues from hybrid pigs was studied by indirect immuno-fluorescence staining techniques.The frozen sections of the pancreas were incubated with the sera from diabetic patient or human controls.and then incubated with FITC-conjugated goat antihuman IgG or IgM monoclonal antibodies.The results showed that all IDDM and human control sera could react to one or the other kinds of the pancreatic cells which include:islet cells,vascular endothelial cells,ductal epithelial cells and macrophages.It is suggest that 1.IDDM patients and healthy persons contain NXAs against porcine pancreatic islets.The humoral immunity might play a major role in rejection in porcine islets xenotransplantation.2.Cross-matching test and pretransplant graft treatment are needed pretransplantly. Key words: Swine; Islands of langerhans; Xenoreactive natural antibody

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