Abstract
Two fixed-bed loop reactors were used to evaluate singleand separated-phase anaerobic treatments of a high strength waste-water from ethanol fermentation. The one-phase system consisted of an anaerobic fixed-bed loop reactor containing both acidogenic as well as methanogenic populations allowing a complete conversion of the carbon source into gaseous end products and biomass. The two-phase system consisted of a second fixed-bed loop reactor operated as a methanogenic unit, which was proceeded by a CSTR for acidification, both connected in series allowing sequential acidogenesis and methanogenesis of the organic components. The reactors were operated under steady state and variable process conditions. By gradually increasing the feed supply in both systems, maximum turnover of COD was determined. The separated-phase system consistently gave a better quality effluent with lower suspended solids and total COD. Maximum loading rates and COD elimination of the methanogenic phase of the two-phase system was over two times higher than that of the one-phase system. Process stability was also higher. On overloading the methane reactor of the two phase system accumulation of different fatty acids within the reactor was observed. Hydrogen concentration in the biogas can be used as a reliable indicator for system overloadings. At least, continuous online monitoring of hydrogen in the methanogenic reactor gas should provide a convenient alternative to other analyses for process control.
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