Abstract
1. 1. Defatted ragweed pollen was extracted with a number of solvents and the various fractions were analyzed by free and paper electrophoresis and ultracentrifugation. All fractions were compared for their allergenic reactivity by skin tests. 2. 2. Extraction of defatted ragweed pollen with different solvents yielded fractions of different composition. 3. 3. All the extracts soluble in the different solvents displayed allergenic reactivity, while the water-insoluble ragweed was the only one of the insoluble residues to display reactivity consistently. 4. 4. The results of this study suggest that zone electrophoresis is suitable for the further fractionation of the various ragweed pollen fractions.
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