Abstract
AbstractGlucose isomerase was produced from Streptomyces phaeochromogenes by aerobic fermentation at 28°C for 24 h. The crude enzyme was obtained by disintegrating the harvested cells. Ammonium sulphate at a saturation of 0.65 gave the maximum enzyme recovery 89.4%) from the crude extract, while acetone gave 66.4% at a concentration of 3/1 (v/v). The crude enzyme extract was purified by concentration, dialysis, precipitation with ammonium sulphate, passing through a column of Amberlite, and then the eluate was treated with acetone to precipitate the enzyme. The kinetic behavior was studied and it was found that optimum D‐glucose concentration was 0.8‐M. No change in Km value was observed, while Vmax was affected.
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