Abstract
SUMMARYExperiments to elucidate the possible mode of action of MgSO4 with or without 2-deoxy-d-glucose (2DG) in inducing protoplasts in early log phase yeast cells of Histoplasma capsulatum Darling without added external enzymes are described and discussed. MgSO4 is effective in a molarity range between 1.8 and 2.2 M. Below 1.8 M no protoplasts are formed, while 2.4 M MgSO4 produces irreversible plasmolysis. For acceleration of protoplast formation, the optimum concentration of 2DG is 750 mg/100 ml medium. Protoplasts are formed at glucose concentrations ranging from 0.1% w/v to 1.25% w/v. Glucose concentrations of 0.1% w/v occasionally yield multiple budding whole yeast cells.
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