Abstract

Determination of the lipid productivity and composition of 33 strains of the genus Mortierella using glucose or decane as the carbon source was carried out. The maximum accumulation of lipids was 2.01g/400ml medium (total intracellular lipids/dry cell weight, 49.7%) in the M. isabellia IFO 8308 strain grown at 20°C using glucose as a carbon source. The lipids extracted from 10 day-cultured cells were fractionated into neutral and polar lipid fractions by silicic acid column chromatography. The fatty acid and lipid compositions of both fractions were determined. The content of γ-linolenic acid (all cis-6, 9, 12-octatrienoic acid) in the M. ramanniana var. angulispora IFO 8187 strain was high under each cultural condition using either glucose or decane as the carbon source.

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