Abstract
Studies on Positive and Negative ionization mode of ESI-LC-MS/ MS for screening of Phytochemicals on Cassia auriculata (Aavaram Poo)
Highlights
The medicinal plant extracts and phytochemical both with identified antimicrobial properties are of huge importance
Cassia auriculata commonly known as” Avaram” that belongs to the Caesalpiniaceous family[1] and it have great in medicinal properties
In the present study an effort has been made to standardization of solvent system (Methanol, Water, Acetonitrile, Ethyl Acetate, Ethanol, Chloroform, Hexane, Acetone Diethyl ether) and Characterization of flavonoids (Gallic acid, Theanine, Theobromine, Theophylline, Caffeic acid, Caffeine, Ferulic acid, Theacrine, Catechin, Quercetin, EpiGallo Catachin, catechin gallate, Epicatachin gallate and Quercetin hexoside) through positive and negative electrospray ionization mode using LC-mass spectrometry (MS)/MS
Summary
The medicinal plant extracts and phytochemical both with identified antimicrobial properties are of huge importance. The capillary is the negative electrode (cathode) and the sampling aperture plate is the positive electrode (anode) This mode causes negative ions to predominate the sprayed droplet and is used where the analytes form anions in solution. The seeds are used in diabetes, opthalamia and chylous urine.[7] In the present study an effort has been made to standardization of solvent system (Methanol, Water, Acetonitrile, Ethyl Acetate, Ethanol, Chloroform, Hexane, Acetone Diethyl ether) and Characterization of flavonoids (Gallic acid, Theanine, Theobromine, Theophylline, Caffeic acid, Caffeine, Ferulic acid, Theacrine, Catechin, Quercetin, EpiGallo Catachin, catechin gallate, Epicatachin gallate and Quercetin hexoside) through positive and negative electrospray ionization mode using LC-MS/MS. Conclusion: Based on the results the methanol is the suitable extraction solvetnt and negative ionization mode of ESI-LC-MS/MS was appropriate for the screening of flavonoids on Cassia auriculata flower extracts.
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