Abstract
The aim of this study was to investigate the crude extracts isolated from branches of Prunus spinosa L. (Rosaceae), in order to quantify polyphenols and tannins using instrumental methods (UV-Vis spectrometry and HPTLC densitometry). The qualitative analysis on biomass revealed the presence of valuable compounds such as polyphenols, flavonoids and tannins. HPTLC fingerprinting of crude methanol extract showed several peaks, with different Rf values, corresponding to phytocompounds such as chlorogenic acid (Rf 0.52), neochlorogenic acid (Rf 0.58), caffeic acid (Rf 0.95) and protocatechuic acid (Rf 0.96). The quantitative analysis revealed that the branches of P. spinosa contain polyphenols (2.97�0.059%g gallic acid equivalent/g dried vegetal material) and tannins (0.90�0.033%g tannic acid equivalent/g dried vegetal material). The HPTLC densitometry analysis offers information about the amount of neochlorogenic acid in the vegetal material 0.12% g/g. The content in polyphenols and tannins suggests the possibilities to use this species to separate compounds with antioxidant potential. These results open perspectives for complex valorization of Prunus spinosa species, a bioresource known only for phytotherapeutical and nutritional potential of their fruits.
Highlights
The aim of this study was to investigate the crude extracts isolated from branches of Prunus spinosa L. (Rosaceae), in order to quantify polyphenols and tannins using instrumental methods (UV-Vis spectrometry and High Performance Thin Layer Chromatography (HPTLC) densitometry)
This study presents the results of investigating polyphenols and flavonoids separated from P. spinosa branches using different extraction solvents and applying various qualitative and quantitative methods
Intense absorption bands corresponding to stretching vibration of functional groups specific to polyphenols and flavonoids (OH group 3279 cm-1, C-H aromatic 2903 cm-1, C-C from aromatic ring 1726 cm-1, CO and OH group from polyphenols 1235 cm-1) were detected using FT-IR spectroscopy
Summary
The aim of this study was to investigate the crude extracts isolated from branches of Prunus spinosa L. (Rosaceae), in order to quantify polyphenols and tannins using instrumental methods (UV-Vis spectrometry and HPTLC densitometry). The aim of this study was to investigate the crude extracts isolated from branches of Prunus spinosa L. The content in polyphenols and tannins suggests the possibilities to use this species to separate compounds with antioxidant potential. Different flavonoids have been isolated from the leaves of P. spinosa such as kaempferol, quercetin, their 3-O-α-Larabinofuranosides, kaempferol 3- and 7-O-α-Lrhamnopyranosides [3] The blackthorn constituents, such as flavonoid pentosides (arabinosides, xylosides, rhamnosides) and A-type procyanidin dimers with twicebonded structures are quite rare in nature [4]. The aim of this study was to investigate the polyphenols composition of branches of P. spinosa in order to identify and quantify some bioactive compounds with antioxidant potential. Qualitative analysis Phytochemical study: Characterization of P. spinosa extracts for various phytochemical constituents was carried out using standard methods [7] as described in table 1
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