Studies on Polynucleotides: CXXII. THE DODECANUCLEOTIDE SEQUENCE ADJOINING THE C-C-A END OF THE TYROSINE TRANSFER RIBONUCLEIC ACID GENE

Abstract

Abstract Primer-template complexes were prepared by annealing synthetic deoxyribopolynucleotides with nucleotide sequences corresponding to the 3' end of transfer ribonucleic acidtyr to the r strand of φ80psuiii DNA, which contains the tRNAtyr gene (2). Using these systems, the nucleotide sequence beyond the 3' end of the primers was determined by the DNA polymerase I-catalyzed addition of nucleotides by using a restricted number of deoxyribonucleoside 5'-triphosphates. In agreement with Altman and Smith (3), it was found that the sequence C-C-A is encoded in the tRNAtyr gene. The sequence of the 12 nucleotides following the C-C-A end was shown to be T-C-A-A-C-T-T-T-C-A-A-A.