Abstract

Abstract Humic acid extracted from a Brunizem soil was purified using cationic and anionic exchange resins and EDTA. The purified humic acid was passed through a column of weakly acid cation exchange resin saturated with iron labelled with 59Fe. The Fe‐humate was eluted from the column at pH 2.7. The rate of iron absorption by excised barley roots from solutions of Fe‐humate was studied. Iron absorption by the roots remains steady during the first eight hours. Absorption from solutions of different concentrations showed the presence of a single pattern between 7.14 x 10‐7 M to 2.6 x l0‐4 M iron concentration range. Metabolic inhibitors and low temperature produced only minor effects. Absorption of iron by roots of entire sunflower plants and transport to the shoot was also studied. The results showed that absorption and transport of iron from Fe‐humate was lower than the absorption and transport of iron from FeEDTA or FeCl3. Absorption of iron from Fe‐humate by leaves really occurs but no transport out of...

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